Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAA-APDVFFTEFTNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDINMGCPVPNVAERGKGSGLILRPEVAAQLIEAAKA-GGLPVSVKTRIGYTEM---SEMEEWISHLLHQDIANLSVHLRTRKEMSKV-------DAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGEELAEKYGVDGIMIGRGIFKNPYAFEKDPRE----HTEKELIGLLRLQLDLQDHYSEIIPRSITGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ 3B0U Chain:Y ((2-313)) ---------LDPRLSVAPMVDRTDRHFRFLVRQVSLGVR-LYTEMTVDQAVLRGN--RER--LLAFRPEEHPIALQLAGSDPKSLAEAARIGEAFGYDEINLNLGCPSEKAQEGGYGACLLLDLARVREILKAMGEAVRVPVTVKMRLGLEGKETYRGLAQSVEAMAEAGVKVFVVHARSALLALSTKANREIPPLRHDWVHRLKGDF----PQLTFVTNGGIRSLEEALFHLK--RVDGVMLGRAVYEDPFVLEEADRRVFGLPRRPSRLEVARRMRAYLEEEVLKGT-PPWAVLRHMLNLFRGRPKGRLWRRLLSEGRSLQALDRALRLME----------

General information: TITO was launched using: RESULT: Template: 3B0U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Y - contact count / total energy / energy per contact / energy per residue : 1601 7611 4.75 25.71 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain Y : 0.69 3D Compatibility (PKB) : 4.75 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_3B0U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B0U-query.scw PDB file : Tito_Scwrl_3B0U.pdb: