Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -RRMCILTLAEKTQNPEK---PNFLEDTYQ-EVEAPAAAMLS-------YGGHEIHFE-LDKNCWPRLR---HGSAVPPGYSAHIYSEGSDKYPPTSAVIEGTHVQK-----LPGFNPKGNVRFYEKLIGLS--- 4Q7O Chain:A ((4-136)) MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNITTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDD-FNPEELIIPINLAYHYFNIAISDFLIA-HPEYQKKCKEIQKTY

General information: TITO was launched using: RESULT: Template: 4Q7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 423 12042 28.47 110.48 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 28.47 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_4Q7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q7O-query.scw PDB file : Tito_Scwrl_4Q7O.pdb: