Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------EDTKHKCFIDIWKQEGDWTRGRESL-YLSVAKNPGESYWFKQGLLGASFTLITIDDDCDASLRFGKMPPRFFFEGRENSMYGLTGHKLIGEINYQKRHPFQNTR- 4G6V Chain:B ((2-104)) AIDLFCYLSIDRGAAESDLNKIRSNHSELFEGKFLISPVRDADFSLKEIAAEHGLVAESFFLVSLND--KNSADLIPIVSKILVDGFNG-----------GAILILQDNEYRRTSL

General information: TITO was launched using: RESULT: Template: 4G6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 345 24572 71.22 273.02 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : 71.22 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.135

(partial model without unconserved sides chains): PDB file : Tito_4G6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G6V-query.scw PDB file : Tito_Scwrl_4G6V.pdb: