Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVDLFHAMRVFNKVVETNSFSLAADSLGLPRASVTTTIQALEKHLQVRLLNRTTRKISLTPDGAVYYDRTARILADVADIESSFHDAERGPRGQLRIDVPVSIGRLILIPRLRDFHARYPDIDLVIGLN--D-RPVDLVGEAVDCAIRVGELKDSSLIARRIGTFQCATAASPIYLEKY-GEPTSIEDLQKNHKAIHFFSSRTGRNFDWDFVVDDLIKSVSVRGRVSVNDGDAYIDLALQGFGIIQGPRYMLTNHLESGLLKEVLPQWTPAPMPISAVYLQNRHLSLKVKVFVDWVAELFAGCPLLGGTALPFDQKCEFACDKETGHEYTIRTLVEQHNIAEAYTLKT 3ISP Chain:A ((5-296)) --QLDGPQLAALAAVVELGSFDAAAERLHVTPSAVSQRIKSLEQQVGQVLVVRE-KPCRATTAGIPLLRLAAQTALLESEALAEMG--ASLKRTRITIAVNADSMATWFSAVFDGLGD----VLLDVRIEDQDHSARLLREGVAMGAVTTERNPVPGCRVHPLGEMRYLPVASRPFVQRHLSDGFTAAAAA-KAPSLAWNRDDGL--QDMLVR-KAFRRA-ITRPTHFVPTTEGFTAAARAGLGWGMFPEKLAASPLADGSFVRVCD--I--HLDVPLYWQCWKLDSPIIARITDTVRAAASGLYRGQ-----------------------------------------

General information: TITO was launched using: RESULT: Template: 3ISP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1411 -79552 -56.38 -278.15 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -56.38 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_3ISP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ISP-query.scw PDB file : Tito_Scwrl_3ISP.pdb: