Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAQALVLTDNAANKVRQLRDSEGNDDLMLRVYV--------TGGGCS-GFSYGFNFAES---I-NEDDAEFVNGDVKMLVDSLSYQYLV-GSVVDYVEGLEGSRFIVQNPNATTTCGCGSSFSI 2P2E Chain:A ((3-106)) ---LKITVTDDAAKKLQRYTDDS---NAVLLLDFDDGVGALSKVGVCSLNSDFRILVVSKDMDYKKDYNEVIDSNIGKFYYKGYSKMYMDDNMKISLNTN--NSLLRLTGDN-------------

General information: TITO was launched using: RESULT: Template: 2P2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 369 -9723 -26.35 -108.03 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -26.35 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_2P2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P2E-query.scw PDB file : Tito_Scwrl_2P2E.pdb: