Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTESTTPKFAIPELPMQIQTIRQYLPHRYPFLLVDRVTEVTDN-SIIGYKNVSINEEFLQGHFPEYPIMPGVLIVEALAQVSGVLGFIMNNETPKPGSLFLFAGAERVRFKKQVVAGDQLVLKSELVMQKR--GIYKYNCTASVDGIVAATAEIMISHQKTEQA 3AZB Chain:O ((11-141)) ---------------IDIEDIKKILPHRYPFLLVDKVIYMQPNKTIIGLKQVSTNEPFFNGHFPQKQIMPGVLQIEALAQLAGIL--CLKSDDSQKNNLFLFAGVDGVRWKKPVLPGDTLTMQANLISFKSSLGIAKLSGVGYVNGKV----------------

General information: TITO was launched using: RESULT: Template: 3AZB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 520 -41437 -79.69 -323.72 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain O : 0.79 3D Compatibility (PKB) : -79.69 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_3AZB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AZB-query.scw PDB file : Tito_Scwrl_3AZB.pdb: