Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDSQQSKPKHVMMMAAGTGGHVFPALAVAKQLQQQGCQVSWLATPIGMENRLLKDQNIPIYQIDIQGV-RGN------GVI-------RKLA----A-PFK-ILKATFSAMRYMKQLKVDAVAGFGGYVAGPGGLAARLLGIPVLIHEQNAVAGF---------TNAQLSR-------VAKVVCEAFPNTF--PA-SEKVVTTGNPVRREITDILSPKWRYDEREQAGKPLNILIVGGSLGAKALNERLPPALKQLQVPLNIFHQCGQQQVEATQALYADAPANLTVQVLPFIEDMAKAYSEADLIICRAGALTVTEVATAGVAAVFVPLPIAVDDHQTANAKFLADVGAAKICQQSTMTPEVLNQLFTTLMNRQLL-TEMAVKARQHAQPNATQHVVGLIQKM 4G2T Chain:A ((14-396)) --------HMRILVIAGCSEGFVMPLVPLSWALRAAGHEVLVAASENMG--PTVTGAGLPFAPTCPSLDMPEVLSWDREGNRTTMPREEKPLLEHIGRGYGRLVLRMRDEALALAERWKPDLVLTETYS--LTGPLVAATLGIPWIEQSIRLASPELIKSAGVGELAPELAELGLTDFPDPLLSIDVCPPSMEA--GTTKMRYVPYNGRNDQVP---S----WVF-EERKQPRLCLTFGTRVPL-LLQALSQELPKL--GFEVVVA-VSDKLA---------PEG--VLAAGQF-PLSAIMPACDVVVHHGGHGTTLTCLSEGVPQVSVPVIA----EVWDSARLLHAAGAGVEVP-------SVLAACARIRDDSSYVGNARRLAAEMATLPTPADIVRLIEQ-

General information: TITO was launched using: RESULT: Template: 4G2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1775 -106393 -59.94 -337.75 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -59.94 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_4G2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G2T-query.scw PDB file : Tito_Scwrl_4G2T.pdb: