Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMRTHYCGSLTEAQIDQTVTLCGWVHRRRDHGGVIFLDMRDRDGLVQVVIDPDTPEAFATADKARSEYVLKITGRVRRRYEGTENPNMVSGQIEVLGKEIEVLAASETPPFPLNDDTINVSEEHRLKYRFLDIRRPEMLDRLRFRSKVTNLIRNYLDDHGFLDVETPILTRATPEGARDYLVPSRVQNGSFYALPQSPQLFKQLLMVGGIDRYYQIAKCFRDEDLRADRQPEFTQIDIETSFLN-DDDIMDLMEGMTVKLFNDLLGVKFE-KFRRMPYSEAMRDYASDKPDLRIPLKLVDVADLMQEVEFKVFAGPAKDPKGRIAALRVPGAGSLTRSQIDEYTKFVGIYGAKGLAYIKVNEIEKGIEGLQSPIVKFIEP-IVMQLLERVGAENGDIVFFGADKAKIVNDAMGALRVKIGHDLNLA-TCEWAPLWVVDFPMFEEAD-----------DGKWTSVHHPFTLPKS-SVEDVKSNPGEALSVAYDMVLNGTEVGGGSLRIYTLEMQKAIFEALGISDEEAEEKFSFLLNALRYGAPPHGGLAFGLDRLVMLMTGATSIRDVIAFPKTKTAECPLTQAPAPVEANQLRDLGIRLREQQKKEA 4RMF Chain:A ((2-580)) -LRTHAAGSLRPADAGQTVTLAGWVARRRDHGGVIFIDLRDASGVSQVVFREGDV--LAAAHRLRAEFCVAVTGVVEVRPEGNENPEIPTGQIEVNATELTVLGESAPLPFQLDE---QAGEEARLKYRYLDLRREGPGNALRLRSKVNAAARSVLAEHDFVEIETPTLTRSTPEGARDFLVPARLQPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEMSFVEAD-DVIAISEQVLKAVWATIG-YDLPLPLPRISYEEAMRRFGSDKPDLRFGIELVECTEYFKDTTFRVFQA------PYVGAVVMPGGASQPRRTLDGWQEFAKQRGHKGLAYVLVGED----GTLGGPVAKNLSDAERDGLVAHVGANPGDCIFFAAGPAKGARALLGATRIEIAKRLDLIDPNAWAFTWVVDFPMFEAADEATAAGDVAVGSGAWTAMHHAFTAPKPDSVDTFDSDPGNALSDAYDIVCNGNEIGGGSIRIHRRDIQERVFAMMGIDHDEAQEKFGFLLDAFSYGAPPHGGIAFGWDRITALLAGVDSIREVIAFPKSGGGVDPLTDAPAPITPQQRKESG-----------

General information: TITO was launched using: RESULT: Template: 4RMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2839 -42166 -14.85 -74.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -14.85 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_4RMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RMF-query.scw PDB file : Tito_Scwrl_4RMF.pdb: