Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSIFDLHIQVQPQHIDALGHVNNVMYVQWMQDVAAAHVETLGVGVTKYLE-LKHAMVAVEHHVQYRKAAFEGEEIVLRTWLDDINALY-SFRQYAFFRPSDQAILFVGNTKWACIEIASGRPKRMSPTFTQAYKPLDSSINPLDFTVSYA 4K00 Chain:A ((5-140)) --GTFTYERQVYLADTDGAGVVYFNQFLQMCHEAYESWLSSEHLSLQNIISVGDFALPLVHASIDFFAPAHCGDRLLVNLTITQ-ASAHRFCCDYEISQAESAQLLARAQTHHVCIALPERKKAPLPQPWQTAICDLDH------------

General information: TITO was launched using: RESULT: Template: 4K00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 611 -44072 -72.13 -328.90 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -72.13 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_4K00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K00-query.scw PDB file : Tito_Scwrl_4K00.pdb: