Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWNPNSIIFCFYFIFSILALWGIIEAWVHQSRTETIHPFKAFVHLLAFYLSYLLIPLWFFNLYAGWIGYYSIHETIFIFLLSGILIYARFIEPHMVRVDVHQYRLNPDRHFVKPVKVALIADLHIGLFSGHERQLKIIVKKLNEQQPDLVVVAGDWTYEPEDK-------------LVQELSVLK---DIKAPVYSVPGNHDEQYPGPPIQQLLKDALYYNDVMDIEGK-IVEFDEFRLIGIGDLWAGK-------------T----------DMRSMPDL-PQDKPWLILSHNPDTVDM--------------------------V---PKLPNRPLMLSGHTHGGQVELPLVTNYIMKKVSILGHKRGFYSHEHADVFVTVGTGMVGIPLRFRVPPTIDIIELA 5K73 Chain:D ((6-239)) ---------------------------------------------------------------------------------------------------------------EQIQHIAIVGCVHGKYREMY-RQLSEYEKS-TGKEISFVICTGDMQTLRYEADLVYLKVPPKYKQMGDFHLYYEGKEKAPYLTLFIGGNHESSNVLLHL---YNGGFVCFNMYYLGVCSCININGLRIVGVSGIYKSFDEKKPYTYPPSPNDVVSLFHTRNYVIQMLSNLSQSSQIDISLSHDWPQGIVMKGNYKQLYRFQPGFKKDGASLGSPINKVILNTLKPKYWISGHMHCEYHA--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5K73.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 761 -51266 -67.37 -312.59 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.53 3D Compatibility (PKB) : -67.37 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_5K73.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K73-query.scw PDB file : Tito_Scwrl_5K73.pdb: