Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLPLYVTSGEPAGIGPDICLSLAKRV--DERPVVVLADRNLLHQRAQKL-GLDIKFLEYSGQAESS--LQGELYIEHV-P-LETIVVDGQLNAANAAYVLEQLRRSADYAMSGKSVGVATAPVQKSVINDAGILFSGHTEYYQEFAGVERVVMMLATKTLRVALATTHLPLRDVADAITKERLHQVIDILLRDLKTKFKINDPRVLVCGLNPHAGEDGYLGREEIDTINPVLESYRSQGVKLSLSLPADTLFTPEHLKNADAVLAMYHDQGLPVLKSQGFGEAINITLGLPFIRTSVDHGTALSLAGTGLAKSSSLNVAADLALSLAAS 2HI1 Chain:A ((3-327)) TKTVAITMGDPAGIGPEIIVKALSEDGLNGAPLVVIGCLATLKRLQAKGITPNVELRAIE-RVAEARFAPGIIHVIDEPLAQPEALEAGKVQAQAGDLAYRCVKRATELALRGDVQAIATAPLNKEALHLAGHNYPGHTELLATLTHSRDYAMVLYTDKLKVIHVSTHIALRKFLDTLSTARVETVIGIADTFLKR-VGYVKPRIAVAGVNPHAGENGLFGDEETRILTPAITDARAKGMDVYGPCPPDTVFLQAYEGQYDMVVAMYHDQGHIPLKLLG-YDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIKLAMQLA--

General information: TITO was launched using: RESULT: Template: 2HI1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1820 8002 4.40 25.24 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 4.40 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_2HI1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HI1-query.scw PDB file : Tito_Scwrl_2HI1.pdb: