Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLSASLQAIDIDDSIQLEQDGSILYLWLNRPESRNAMNLNMVNAIQQVFTAIRDDLSIRAVIIRGEGGTFCAGGDIKDMAALRVEATNVGSLQPYTDFNRRFGAMLEQVEAAPQTVVVILEGAVLGGGFGLACVSDVAISRDNAQFGLPETGLGVIPAQIAP-FVVKRIGLTQARRLALLGMRFEGHTALSVGVVHQIAHNEIELEQALHETIQHIKRAAPQASRVTKALLHRTLNEPLNELLDDAAQQFAQAVGGAEGQEGTMAFIQKRLPNWADES 3H81 Chain:C ((23-278)) -----------TYETILVERDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAGADIKEMADLT----------FADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPAD-DLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQSEGMAAFIEKRAPQFTHR-

General information: TITO was launched using: RESULT: Template: 3H81.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1311 -115519 -88.12 -453.02 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -88.12 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_3H81.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H81-query.scw PDB file : Tito_Scwrl_3H81.pdb: