Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN----PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIGYNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTFAQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLDISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPIHVIEKLFD 2EV9 Chain:B ((10-244)) --------------PVAHSLSPAMHAFALESLGLEGSYEAWDTPLEALPGRLKEVRRAFR-----GVNLTLPLKEAALAHLDWVSPEAQRIGAVNTVLQVEGRLFGFNTDAPGFLEALKAGGIPLKGPALVLGAG--GAGRAVAFALREAGLEVWVWNRTPQRALALAEEF---GLRAVPLEKAR-------------EARLLVNATRVGL-EDPSASP-LPAELFPEEGAAVDLVYRPLWTRFLREAKAKGLKVQTGLPMLAWQGALAFRLWT--------------

General information: TITO was launched using: RESULT: Template: 2EV9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1263 -2883 -2.28 -12.48 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -2.28 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_2EV9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EV9-query.scw PDB file : Tito_Scwrl_2EV9.pdb: