Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PHFCEVSVSYENANKQKITTATKYVVPPGSVDIAV-GSQMYAVTVD-ETCKRTDSISIPG-RFKIDTQGKKLEGNP---------------------TENYIR--------SVVLQLNHRGLRMVSRPAP-- 2LVL Chain:A ((1-128)) ----GSTMHFTDDNENDTSETMESLIDKGKLDQVVYDDQLYHLKEKVDEDKKGKVIGAIGQTFFVDGDGKRWSEEELKEPYISNNPDEIREKKPLRYGKVYSTNEDSDAKDEIIVEFNREYYRAVLIKNEKE

General information: TITO was launched using: RESULT: Template: 2LVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 202 4439 21.98 47.22 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 21.98 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_2LVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LVL-query.scw PDB file : Tito_Scwrl_2LVL.pdb: