Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------SLLSTISNDKCFVSSY---KFTPDGRKVIEASQAAPGDGPITFTST-RDNFHMTIQ-IDANCAPRNPDDIQKELPSHRGFDFYTKAHNLPG------LNPRHN- 3F1Z Chain:A ((15-133)) QPGDDAVASMQTYSVAQFLQPFTLNPAKASSDYLGKWVKVRGVIVDIRRKSGIAGSYYFIVTMRDEQNKTDKRLTFNFGSHNSADVEA-LSNGSVATIVGQVHQVQDSTIPTLQNPKVVK

General information: TITO was launched using: RESULT: Template: 3F1Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 274 16365 59.72 179.83 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 59.72 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.079

(partial model without unconserved sides chains): PDB file : Tito_3F1Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F1Z-query.scw PDB file : Tito_Scwrl_3F1Z.pdb: