Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------YGDCFIGLYEKGGRNPNHIAGTEADESGTFEVL---HENYKIRVKIF---KCVKQHVYDSTPSG----TEIRVIPS----VGYDPNKRQTAPRPAPRPAPRPAPRRTQNAAGHR--------------- 1A81 Chain:A ((1-129)) SANHLPFFFGN--ITREEA-----EDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIER-ELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPFEDLKENLIREYVKQTWN

General information: TITO was launched using: RESULT: Template: 1A81.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 355 13042 36.74 141.76 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 36.74 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_1A81.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A81-query.scw PDB file : Tito_Scwrl_1A81.pdb: