Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------PEHC--FVRILKYEIPIHGDG----YLVGSDVNWWVGNQKVDFHINNDCTIQRRGGTQEKKPYRLDSISTLGEDLPKGFDFILFRESKIDEFLAQGGVTKRILGSMIL------------- 1JFL Chain:A ((1-115)) MKTIGILGGMGPLATAELFRRIVIKTPAKRDQEHPKVIIFNNPQ------------IPDRTAYILGKGEDPRPQLIWTAKRLEE---CGADFIIMPCNTAHAFVED--IRKAIKIPIISMIEETAKKVKELG

General information: TITO was launched using: RESULT: Template: 1JFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 299 5362 17.93 63.08 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 17.93 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_1JFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JFL-query.scw PDB file : Tito_Scwrl_1JFL.pdb: