Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKVK 5VBT Chain:A ((2-78)) MPIFVKTLTGKNITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFTGKKLEDGRTLSDYNIKFASTLHLVIRLRGAR---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5VBT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 315 -39929 -126.76 -518.55 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -126.76 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.760

(partial model without unconserved sides chains): PDB file : Tito_5VBT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VBT-query.scw PDB file : Tito_Scwrl_5VBT.pdb: