Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD 2RCY Chain:C ((5-261)) IKLGFMGLGQMGSALAHGIANANIIKKENLFYYGPSKKN--T------TLNYMSSNEELARHCDIIVCAVKPDIAGSVLNNIKPYL-SSKLLISICGGLNIGKLEEMVGS---ENKIVWVMPNTPCLVGEGSFIYCSNKNVNSTDKKYVNDIFNSCGIIHEIKEKDMDIATAISGCGPAYVYLFIESLIDAGVKNGLSRELSKNLVLQTIKGSVEMVKKSDQPVQQLKDNIVSPGGITAVGLYSLEKNSFKYTVMNAVEAACEKSKAMG---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2RCY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1101 -168472 -153.02 -655.53 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -153.02 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_2RCY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RCY-query.scw PDB file : Tito_Scwrl_2RCY.pdb: