Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSSGPGPRFLLLLPLLLPPAASASDR--PRGRDPVNPEKLLVITV-------------ATAETEGYLRFLRSAEFFNYTVRTLGLG-----------EEWRGGDVARTVGGGQKVRWLKKEMEKY-----ADREDMIIMFVDSYDVILAGSPTELLKKFV------------------QSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNG-------PTKLQLNYLGNYVPN--GW-TPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHN-----NEVFHEPHIADSWPQLQDHFSAVKLVGP---EEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQ-TLRILIEENRKVIAPMLSRHGKLWSNFWGALSP--------DEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCK------SFRDKGIFLHLSNQ----HEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKE--------QYIHENYSRALEGE-----GIVEQPCPDVYWFPLLSEQMCD--------------ELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWL--QLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPD---EQPSLR----PHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVI--SSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 4MT1 Chain:A ((2-1040)) AKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTITLHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENLAQVEVQNKLS--EVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNVVPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIGSLPAVRGQTV---TATVTAQGQLGTAEEFGNVILRA----NTDGSNIYLKDV--AKVGLGMEDYSSSTRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEISIEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLTMFAMILVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISVFVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATGFFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPT-SFLPTEDQGFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFAIFKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGSGLSINLQDRNNTGHTALLAKRNELIQKMRASGLFDPS----TVRAGGLEDSPQLKIDINRAAAAAQGISFADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPLSTIATVSWENGTEQSVRFNGYPSMKLSASPATG---VSTGQAMEAVQKMVDELGGGYSFEWGGQSSEEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVTGRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAALEAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLVGTLLSVFLVPLFYVVVRKFF

General information: TITO was launched using: RESULT: Template: 4MT1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3137 -931 -0.30 -1.29 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -0.30 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_4MT1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MT1-query.scw PDB file : Tito_Scwrl_4MT1.pdb: