Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKPEFLESAEFYNRRYHNFSSSVIVPMALLLV-----------FLLGFATVAEKEMSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKG--DLLVQYQEGAEGVQAESYASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQNETIASQNAAASQTQAEIGNLISQTEAKIR---DYQTAKSAIETGTSLAGQNLAYSLYQSYKSQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKVGQELSLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEGTLLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSIDATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE 5IWZ Chain:A ((4-397)) MPVRPDLQQLEKCIDDALRKNDFKPLLALLQIDICEDVKIKCSKQFLRKLDDLICRELNKKDIQTV--SSILISIGRCSKNIFILGQAGLQTMIKQGLVQKMVSWFENSKEIILNQDEAVMNMIEDLFDLLMVIYDISDEGKNQVLESFIPQICALVIDS-------RVNFCIQQEALKKMNLMLDRINQEMLTLMSNMGERILDVGDYELQVGIVEALCRMTTEKRRQELAYEW-------------------------------FSMDFIANAFKEIKDCEFETDCRIFLNLVNGILGDKRRVYTFPCLSAFLGKYE----LQIPSDEKLEEFWIDFNLGSH------------------------TLSFYIEAVTVPEEK----VQMYNIEVRESKKLLTLTLKNIVKISKKEGKELLFYFD-ESLEITNVTKKVFGGLEHH----------------------

General information: TITO was launched using: RESULT: Template: 5IWZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1630 64244 39.41 181.48 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 39.41 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.218

(partial model without unconserved sides chains): PDB file : Tito_5IWZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IWZ-query.scw PDB file : Tito_Scwrl_5IWZ.pdb: