Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFIEYKIITLFISLKIKKGVFIMKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIKMYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAFADN----Y-IVNAQKGAYADLTELYKKEGKDLYKALD--PAYIKGN-TVNGKIYAVPVAANVA--------SSQNFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPD------VVPFAIGKVFIP--SDNFDYPVANGL--PFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSF---DLQQDTWFVREETVGPADYGNSLLSRVANKDIQIKPITNF--IKK-NQ-----TTQVANFVISNNSKNKEKSMEILNLLNTNPELLNGLVYGPEGKNWEKI----EGKENRVRV-LDGY--KGNT-H----MGGWNTGNNWILYIN-ENV-TDQQIENSKKEL----AEAKESPALGFIFNTD----NVKSEISAIANTMQQFDTAINTGTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK 5G5Y Chain:B ((2-494)) --------------------------------------------------------------PKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQ-SDFAEKRNLDISSGDLPDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHIYSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEM--PKTTDDLIKVLEAFKNGDPNGNGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLV--VGNDGKVDFTADNDNYKEGVKFIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFT-WDKNNV----TGS--NESYDVLPVLAGPSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAP-LQSVQNNWGTYGDDKQQNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLDLIKEYYVPYMSNVNNYP---RVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNG-NIDTEWDDYKKELEKYG-LSDYLAIKQKYYDQYQANKN

General information: TITO was launched using: RESULT: Template: 5G5Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2244 125235 55.81 288.56 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 55.81 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_5G5Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G5Y-query.scw PDB file : Tito_Scwrl_5G5Y.pdb: