Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGR---RIKEEGLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALLMEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREKDGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFN-QMVDKVIVAGRDGVQISTSKKGK 4M8L Chain:B ((12-219)) ---LKKLAATEAAKSITTEITLGVGTGSTVGFLIEELVNYRDKIK------TVVSSSEDSTRKLKALGFDVVDLNYAGEIDLYIDGADECNNHKELIKGGGAALTREKICVAAAKKFICIIDESKKVNTLGNFPLPIEVIPMARSYIARQIVKLGGQPVYREQT----ITDNGNVILDVYNLKIDNPLKLETELNQITGVVTNGIFALKPADTVIMATKDS----------

General information: TITO was launched using: RESULT: Template: 4M8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1212 21336 17.60 104.59 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 17.60 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_4M8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M8L-query.scw PDB file : Tito_Scwrl_4M8L.pdb: