Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRNMFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRELVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFYSNYFEKNIE-LGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEERQNTFTDMMKVGLETLIAE 4D8Y Chain:B ((21-251)) MSVHIGAEKGQIADTVLLPGDPLRAKFIAETYLENVECYNEVRGMYGFTGTYKGKKISVQGTGMGVPSISIYVNELIQSYDVQNLIRVGSCGAIRKDVKVRDVILAMTSSTDSQMNRVAFGSVDFAPCADFELLKNAYDAAKDKGVPVTVGSVFTADQFYND--DSQIEKLAKYGVLGVEMETTALYTLAAKHGRKALSILTVSDHVLT-GEETTAEERQTTFHDMIDVALHSV---

General information: TITO was launched using: RESULT: Template: 4D8Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1355 -137866 -101.75 -599.42 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.88 3D Compatibility (PKB) : -101.75 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_4D8Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D8Y-query.scw PDB file : Tito_Scwrl_4D8Y.pdb: