Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLKAKLEGKTSTKIETAALDADKVEEVIALIGSYKPEAVLNVALPYQDLTIMDACLATGVHYIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSGFDPGVTSVFSAYALKHYF----DEIHYIDILDCNGGDH-----GYPFATNFNPEINLREVSAPGSYWEDGKWVEVEAMSI----KREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSYLTHMKCLENVGLLRTDTINFNG----------------------------------------------------------QEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV-KDGVEKTIYIYNVCDHQECYAE-VGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE-LDPDPFMEALN-EYGLPWVVVENPQMVD 1E5Q Chain:D ((3-447)) TKSVLMLGSGFVTRPTLDVLTDSG---IKVTVACRTLESAKKLSAGVQ-----HSTPISLDVNDDAALDAEVA--KHDLVISLIPYTFHATVIKSAIRQKKHVVTTSYVSP----------------------------AMMELDQAAKDAGITVMNEIGLDPGIDHLYAIKTIEE-VHAAGGKIKTFLSYCGGLPAPESSDNPLGYKFSWSSRGVLLALRNAASFYKDGKVTNVAGPELMATAKPYFIYPGF-AFVAYPNRDSTPYKERYQIPEADNIVRGTLRYQGFPQFIKVLVDIGFLSDEEQPFLKEAIPWKEATQKIVKASSASEQDIVSTIVSNATFESTEEQKRIVAGLKWLGIFSDKKITPRGNALDTLCATLEEKMQFEEGERDLVMLQHKFEIENKDGSRETRTSSLC-EYG-APIGSGGYSAMAKLVGVPCAVAVKFVLDGTISDRGVLAPMNSKINDPLMKELKEKYGIECKEK-------

General information: TITO was launched using: RESULT: Template: 1E5Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1842 -76524 -41.54 -206.82 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -41.54 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_1E5Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E5Q-query.scw PDB file : Tito_Scwrl_1E5Q.pdb: