Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQDTTTASSSSEQNQSSNKTQTSAEVQTNAAAYWDGDYYVKDDGSKAQSEWIFDNYYKAWFYINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGNKWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQKVGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLNVGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENKVDGVIVRLGYS----GKEDKELAHNIKELNRLGIPYGVYLYTYAEN-ETDAENDAKQTIELIKKYN---MNLSYPIYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQA-GYQNVYVYSYRSLLQTRLKH------PDILKHVNWVAAYTNALEWE-----NPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY 5JIP Chain:B ((10-223)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NPLSTFGPDLNEFSRDV-NFLTLA--KNSDFIYLRASGSGTGKLRIDNKFLEFAKECRRLGIPCGAYHFAKPSKDLDSAVIQADQFIDVLQQGFGDGDYGDLFPVLDVETPTDK------SLTTTELVNWIDRFRDRFEEKTRRR-LMLYTGLFFIGLYDDFKVPGKGYPLSDMPLWIAMYTRIPSNPRIPPNVGGWKRWTMWQFTDEGKLDGVGSPVDLNWGP

General information: TITO was launched using: RESULT: Template: 5JIP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 958 3703 3.87 19.09 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : 3.87 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_5JIP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JIP-query.scw PDB file : Tito_Scwrl_5JIP.pdb: