Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHFTFLGTSSGV--PTL------------------SRNVSGLAVRNSKN-KDWILVDAGEGTQHRIQQ-------ARLSLQNLIAICITHVHGDHCYGLVGLLASAGMNARNNPLIVIAPKEIQQWFEITAQLTDLHLPYSIKFIDVNEATRPQQL-TDELFIQAHPLSHR----------------VPSFAFSIYIKSTQKKIDIQALTQLGIPKGDIWGHLKRGYDVEFEGRILKSQDFIKIQNQQIHAIIGGDNDRPE-LLADACKDAQLLIHEATYLQTVLD--------KVGKGPMHSS--AKMV-AEFAEQQSLDNLILTHFSPRHQDKTGQQAIIEEVRQFYKGHFYLANDFDEFTLNETGQLLKIE 4Z7R Chain:A ((1-299)) -MHVVILGSAAGGGVPQWNCRCSICSLAWAGDSRVRPRTQSSIAVSP--DGERWLLLNASPDIRQQIQANPQMHPREGLRHSPIHAVLLTNGDVDHVAGLLTLREG-------QPFTLYATPGILASVSDNRVFDVM-AADVVKRQTIAL-N-ETFEPVPGLSVTLFSVPGKVPLWLEDASMEIGAETETTVGTMIEA-------------------------------------------------GGKRLAYIPGCARVTEDLKARIAGADALLFDGTVLEDDDMIRAGVGTKTGWR-MGHIQMNGETGSIASLADIEIGRRVFVHINNTNPVLIE--DSYER-ASVEARGWTVAHDGLTLDL----------

General information: TITO was launched using: RESULT: Template: 4Z7R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1319 8840 6.70 36.53 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 6.70 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_4Z7R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z7R-query.scw PDB file : Tito_Scwrl_4Z7R.pdb: