Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGKIL----NKFKAWYK---------GDPGDM------RWDPRTDTYVGTREPSKHWTAKVLSYFVDFSLLIAKSIKKHPSAYITQLLAFIA-----ILVS------CFSIYLQYYVDDDEYKRCTIAHTNNQEIALKCKK 1APY Chain:B ((1-141)) TIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACNKLSTFTQFSFMVYNSEKNQPTEEKVDCI-

General information: TITO was launched using: RESULT: Template: 1APY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 616 -2773 -4.50 -24.98 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -4.50 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.190

(partial model without unconserved sides chains): PDB file : Tito_1APY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1APY-query.scw PDB file : Tito_Scwrl_1APY.pdb: