Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLAQRLAAVVLTLGLSAGLVGCGFHLKGTNPTATPLVYKKLSL-ELPAKTDDLETQLKVYLTANGVQLSND-NDAYVLRVLEYTPRRQLL---NGKLTEVLLRLTVTFQIEDRQGNKITEPRTLTAARSYQYDLATVNTENQQESYLQRIVIDDLAQQITRQISANRLPKAQP 3BF2 Chain:A ((30-159)) ------------------------------------LTYRSWHIEGGQ----ALQFPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVT-AVINEYLLILTVEAQVLK-RGEPVGKPMTVSVRRVLAYAD---LGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE----

General information: TITO was launched using: RESULT: Template: 3BF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 484 17871 36.92 148.92 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 36.92 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_3BF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BF2-query.scw PDB file : Tito_Scwrl_3BF2.pdb: