Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGTAMNVKNQAQDRQQILIDLYKQYLLSEITLGQLLSYLRKNVLGLSQEQYANLVGISRRTLTDIEQDKGKLTQSVLDKVFKPLGLKAGLVPTHEHIVSKIIKPNE 3DNV Chain:B ((4-74)) ----------------------FQKIYSPTQLANAMKLVRQQ-NGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAK------------

General information: TITO was launched using: RESULT: Template: 3DNV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 233 -23584 -101.22 -332.17 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -101.22 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.717

(partial model without unconserved sides chains): PDB file : Tito_3DNV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DNV-query.scw PDB file : Tito_Scwrl_3DNV.pdb: