Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKRLIGLFSPDLAIDLGTANTL-IYAPGRGIILNEPTVVAIRHSGSQKIVAAVGLDAKQMLGRTPANISAIRPMKDGVIADFEVTETMLNQFIGKVHEKRLFPPAPRVVVCVPCKSTLVERRAIREAVFNASARDVRLIEEPMAAAIGAGMPVEQACGSMVVDVGGGTTEIAIISLQGCVYADSLRIGGDVFDEQIINYVRKAHGCVIGETTAEIIKKEVGMAVSDGTTLE--IEVRGRNLAEGVPRAITVTSDEITQAISDPLQSIVSAVKSALEQTPPELSSDIAERGIVLTGGGALLRNLDKLLAQETGLPVVVAEDPLTCVTRGGGKVLEFFDDPNHDMLFVG 4CZG Chain:A ((2-327)) ---------ISNDIAIDLGTANTLIYQKGK-GIVLNEPSVVALRNVGGRKVVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRK-GSGNPKVIVCVPSGATAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAP----GLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELASDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLEH------------

General information: TITO was launched using: RESULT: Template: 4CZG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1870 -10236 -5.47 -32.09 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -5.47 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_4CZG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZG-query.scw PDB file : Tito_Scwrl_4CZG.pdb: