Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEFLKAYVQTFKDIVSNSSAFTTLFLSVILYSFFYPTAYKAERAESIPIVIVDEEQSLLTSQVIGQTANSPHVKIVDVTANFLEAEQMVREQKADGILLLPSNLTQSLRRGETGGIGLYLSTTNFLKTKEIGLGLATSIEATLKEYIERFGQRTHFQPALSIHQMPLFNTLSGYGSYIFPAVASLIIHQTIVLGLAMLVASYREQHEKITPIRFAGIFASIFTIGCLGSFYLFGFTLWFNDYPHGGNFVGLLVAVPIFISCVIGLGMLIGSLLDILERAGHIIVFSSVPLFLLTGAAWPHQAMPEWLQWFAWCLPSTHAVQMFVQLNQMGVPLNVVAPKLIFLATIGVIFLITAYSRLKVSK 1T6O Chain:B ((1-19)) -------------------------------------------------------------------------------QDSRRSADALLRLQAMAGI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1T6O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3 -818 -272.67 -43.05 target 2D structure prediction score : 0.84 Monomeric hydrophicity matching model chain B : 0.40 3D Compatibility (PKB) : -272.67 2D Compatibility (Sec. Struct. Predict.) : 0.84 1D Compatibility (Hydrophobicity) : 0.40 QMean score : 0.804

(partial model without unconserved sides chains): PDB file : Tito_1T6O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T6O-query.scw PDB file : Tito_Scwrl_1T6O.pdb: