Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAMLPEWTTACPDWEERIVAKKSLMPCEPLFPQVADVAERIFKELILVDVMGSPKMGDVTLDWVIEFVRAIFGSYNPNTKRRLIREFFLLISKKNTKSTIAAGVMLVALLLNDRLSAELIILAPTKEVADNSFNPIRDFIRADEELSAMINVSEHTKTVTHLGTGATLKVIAAESNAAAGKKASIILIDE----VWLFGKRANAESMFREAKGGLASRPEGCVIYLSTMSDEVPC--GVFKQLLDYARDVRDGIKEDKGFLPLIYEFPKRLVEAGEHLKPEHFYITNPNLGASVDLEYLISEFKKVKDAGEESLRDFLAKHLNIEIGMNLRANRWAGAEYWNKQKHVFGLDQLIEQSDVITIGIDGGGLDDLLGYAVLGRLKKDPRVWWL---WNHAWANK--IALERRKEN---------IPKYDDFKLEGSLTVVDRVGDDIDQLAAIAKKVYDSGKLNKIGLDPLGLGGLLDGLLEAGIPEESMFAVPQGYKLMSYILTTERKLAEGNLYHAGQQLMTWAAGNARVVMVGNGMRITKQESGVGKIDPLIATFNAVALMSMNPEPTNKEYNVFFV 1CS8 Chain:A ((1-220)) ----------------------------------------------------------------------------SLTFDHSLEAQWTKWKAMHNRLYGMNEEGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDN-GGLDSEE-------SYPYEATEESCKYNPKYSVAN-------------DAGFVDIPK-QEKALMKAVATVGPISVAIDAGHESFL-----------FYKEGIYFEPDCSSEDMDHGVL--VVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV--------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1CS8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1166 77538 66.50 364.03 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 66.50 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.57 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_1CS8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CS8-query.scw PDB file : Tito_Scwrl_1CS8.pdb: