Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKFLITGGVKLEGEVRISGAKNAALPLLAAMILADSPITLTNVPNLKDVNTLVKLIGGLGVTISYENDTVKADTSTLDNQFAPYELVKTMRASILVLGPLLAHYGNAKVSLPGGCAIGSRPVDQHLKALEALGAHIEVENGYVHATVDGRLKGGEVVFDMVTVGGTENILMAAALADGVTTIRNAAREPEITDLAQMLIKMGAKIEGLDTDTLVVTGVE-SL-HGCEYAVVADRIETGSYLAAAAITGGRVKTTHTDPSLLEAVLDKFEEMGAEVTRGDDWIELDMLGKRPKAVSFRTLPHPEFPTDMQAQIMAVNAIGRGFATISETIFENRFMHVPELSRMGANIQVEGHDAVVTGVEKLQAAPVMATDLRASFSLVLAALVAEGDTLIDRIYHIDRGYEHVEEKLQGLGAKIKRVS 5UJS Chain:B ((25-440)) MTYLEIEGTNHLSGNVTISGAKNAALPLIVSSILAKNEVKINNVPNVADIKTLISLLENLGAKVNFQNNSALLNTNTLNQTIAKYDIVRKMRASILTLGPLLARFGHCEVSLPGGCAIGQRPIDLHLLALEKMGANIQIKQGYVVASG--NLKGNEILFDKITVTGSENIIMAAALAKGKTKLLNVAKEPEVVQLCEVLKDAGLEIKGIGTDELEIYGSDGELLEFKEFSVIPDRIEAGTYLCAGAITNSKITLDKVNATHLSAVLAKLHQMGFETLITEDSITLLP-AKEIKPVEIMTSEYPGFPTDMQAQFMALALKANGTSIIDERLFENRFMHVSELLRMGADIKLNGHIATIVGGKELNAADVMATDLRASSALILAALAAKGTSKVHRIYHLDRGYENLEEKFKDLGAKITRL-

General information: TITO was launched using: RESULT: Template: 5UJS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2460 -120130 -48.83 -290.17 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -48.83 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_5UJS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UJS-query.scw PDB file : Tito_Scwrl_5UJS.pdb: