Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPYT--------------------------------- 4YGO Chain:D ((2-173)) NSQLTLRALERGDLRFIHNLNNNRNIMSYWFEEPYESFDELEELYNKHIHDNAERRFVVEDAQKNLIGLVELIEINYI-------HRSAEFQIIIAPEHQGKGFARTLINRALDYSFTILNLHKIYLHVAVENPKAVHLYEECGFVEEGHLVEEFFINGRYQDVKRMYILQSKYLNRSE

General information: TITO was launched using: RESULT: Template: 4YGO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 460 -4037 -8.78 -32.04 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain D : 0.60 3D Compatibility (PKB) : -8.78 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_4YGO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YGO-query.scw PDB file : Tito_Scwrl_4YGO.pdb: