Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKNMVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNFAESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGSNWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD 2BM5 Chain:B ((6-183)) ------------------------------QWVDCEFTGRDFRDEDLSRLHTERAMFSECDFSGVNLAESQHRGSAFRNCTFERTTLWHSTFAQCSMLGSVFVACRLRPLTLDDVDFTLAVLGGNDLRGLNLTGCRLRETSLVDTDLRKCVLRGADLSGARTTGARLDDADLRGATVDPVLWRTASLVGARVDVDQAVAFAAAHGLCL--

General information: TITO was launched using: RESULT: Template: 2BM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1059 -139803 -132.01 -785.41 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -132.01 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_2BM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BM5-query.scw PDB file : Tito_Scwrl_2BM5.pdb: