Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASFDQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLLEHQQVSIVRLATAFNISESSVFRKIK-ELNQLLEEFGLQIKNGQLYGEELQIRYFYYELFQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKSTYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEIFYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQI-SQINNEWFFFVGKI-EVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVLLMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITFCQRNKKQCEYYLSEFASP------YDIIRLKRRIETLKKEKN-- 4O6D Chain:A ((19-377)) --LYFQSNADTGCAIDISRQELRCGSGVFIH---NDVEAWMDRYKYYPETPQGLAKIIQKAHKEG--------------------------------------------VCGLRSVSRLEHQMWEAVKDELNTLLKENGVDL--------------------SVVVEKQEGMY--KSAPKR-----LTATANNTFVVDGPETKECP---TQNRAWN-------------------------SLEVEDFG--------------------FGLTSTRMFLKVRESNTTECDSKIIGTAVKNNLAIHS-----------DLSYWIESRLNDTWKLERAVLGEVKSCTWPETHTLWGDGILESDLIIPVTLAGPRSNHNRRPGYKTQN--QGPWDEGRVEIDFDYCPGTTVTLS-ESCGHRGPATR---------------TTTESGK---LITDWCCRS---CTLPPLRYQTDSGCWYGMEIRPQRHDEKTLVQSQVNA

General information: TITO was launched using: RESULT: Template: 4O6D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1683 40067 23.81 122.53 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 23.81 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.176

(partial model without unconserved sides chains): PDB file : Tito_4O6D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O6D-query.scw PDB file : Tito_Scwrl_4O6D.pdb: