Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGV-HPAPEYLFVVFCTPVGAYFKGG-LKPTNFIVSD-YDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEVGAANFF--GITKDG--KFITPQ-SPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAEAG------ACGTAAVISPIGGI-YHNNNLHVFYSETEVGP-VTKRLYDELTGIQFGDIEAPEGWIEVVE 2HG8 Chain:B ((39-355)) -------------------------------LMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPD-AAGTSLYVRPVLIGNEPSLGVSQPRRALLFVILC-PVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDH-QLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTAAQVCPVHRILYKDRNLHI--PTMENGPELILRFQKELKEIQYG-------------

General information: TITO was launched using: RESULT: Template: 2HG8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1513 -82296 -54.39 -273.41 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -54.39 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_2HG8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HG8-query.scw PDB file : Tito_Scwrl_2HG8.pdb: