Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERMKKSLVKLVGE--EAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGN-VSDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAKASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVTIEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS 1C14 Chain:B ((2-254)) -GFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKLKGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDGDYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMGLAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRRTVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIA

General information: TITO was launched using: RESULT: Template: 1C14.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1440 -92605 -64.31 -370.42 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.89 3D Compatibility (PKB) : -64.31 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_1C14.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1C14-query.scw PDB file : Tito_Scwrl_1C14.pdb: