Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSGVATLKKYFDGS-VVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGD-----YFKNEAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWDIGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDN-------TFEPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGEN-----------------------------------------------PVTDISYKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVVGEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGKDIFKLYDTYGFPVELTEEVAEDEGLKVDHAG-FEKEMDAQRERARSARSTEKSMGVQSALLTDIK-VESKFIGYTEQ---ESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQVADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIKNHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKIWEAIPVETIETDIDTAKNM-GAMALFGEKYG-HDVRVVNIGGWSIELCGGTHVANTENIGIFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQLQQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQWKQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMAQAGGKNPAGIPDALKAAKEWVAQQ 2ZTG Chain:A ((58-739)) -KPFELDEMREYYLNFFERRGHGRIERYPVVARWRTDIYLTIASIADFQPFVTSGVAPPPANPLTISQPCIRLDDLDSVGRTGRHLTLFEMMAHHAFNYPGKEIYWKNETVAYCTELLN---ELGVKKEDIV------------------Y---------KEEPWA-GGGNAGPCLEAIVG-----------------------GLEVATLVFMNLEEHPEGDIEIKGARYRKMDNYIVDTGYGLERFVWASKGTPTVYDA-IFPEVVDTIIDNSN-VSFNREDERVRRIVAESSKLAGIMGELRGERLNQLRKSVADTVGVSVEELEGIVVPLEKVYSLADHTRCILFMLGDGLVPSNAGAGYLARLMIRRSLRLAEELELG-LDLYDLVEMHKKILGF---EFDVPLSTVQEILELEKERYRTTVSKGTRLVERLVER-----KKKLEKDDLIELYDSHGIPVELAVGIAAEKGAEVEMPKDIYAELAKRHSKAEKVQEKKI------TLQNEYPATE--KLYYDDPTLLEFEAEVIGVE------------GDFVILNRSAFYPESGGQDNDVGYLIANG--GKFEVVDVLEAD-GVVLHVVKG-AKPEVGTKVKGVIDSDVRWRHMRHHSATHVLLYSLQKVLGNHVWQAGARKEFSKARLDVTHFRRPSEEEIKEIEMLANREILANKPIKWEWMDRIEAERKFGFRLYQGGVPPGRKIRVVQVG-DDVQACGGTHCRSTGEIGMLKILKVESIQDGVIRFEFAAGEA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3145 54005 17.17 87.81 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 17.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_2ZTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZTG-query.scw PDB file : Tito_Scwrl_2ZTG.pdb: