Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSNSSVIDAMCYNNSVKSKMKWRKDMDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGVLPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDEVMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDEVTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEVAQAVKNGTIDDCLLPIEK-GVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIALFYKGKVVSIYQPNPKEK---NKLKPSKVLRNEE 1SGV Chain:B ((3-294)) ------------------------ATGPGIVVIDKPAGMTSHDVVGRCRRIFATRRVGHAGTLDPMATGVLVIGIERATKILGLLTAAPKSYAATIRLGQTTSTEDAEGQVLQSVPA-KHLTIEAIDAAMERLR----------------------------SVQLEARPIRIDRFELLAARRRD---QLIDIDVEIDCSSGTYIRALARDLGDALGVGGHVTALRRTRVGRFELDQARSLDDLAERP------ALSLSLDEACLLMFARRDLTAAEASAAANGRSLPAVGIDGVY-----AACDADGRVIALLRDEGSRTRSVAVLRPAT------

General information: TITO was launched using: RESULT: Template: 1SGV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1411 -109840 -77.85 -422.46 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -77.85 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_1SGV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SGV-query.scw PDB file : Tito_Scwrl_1SGV.pdb: