Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQ--EYQLELYE---DAVFLFCG---------NRQDRFKLLYWDGD-GFLLCYKRIENGKLKW-PRTKDEVRTLTNQPVKWLLEGLSIDQPRAILPGKKGVF--- 5TPM Chain:A ((1-155)) SNADLLETRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEAAHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEA-IMAGKPEEAREASHRHLAFIEEIL-LDRSREESRRERSLRRLE

General information: TITO was launched using: RESULT: Template: 5TPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 366 6774 18.51 58.90 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 18.51 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_5TPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TPM-query.scw PDB file : Tito_Scwrl_5TPM.pdb: