Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQERLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQD-FAKVMDKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKLSEAGFSEPE---NWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLDDE-GEVTIDTEKMREALSFYQNLSQY-T-MPGS--NDVTEIKDAFMNGTAPMAIYSTYILPSVYEAGNSENIGFA-IPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKWVLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKMGDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK 5F7V Chain:A ((11-393)) -----------------------------DKTEITYYQFSAP---GKALDEMVKEFEKQNPDIKVNVQTIAFNDYFTKLQTQIAGGDAPDAFELNYETFMQYAEKGVLADLTSYIEKDKDFDPSTLNKQAYDAF-KYDGKQY-GMVESFSNVVTIYNKDLFDKAGVEYPTADWTWKDEEAAAKKLTDAKNKVWGTSQPVT-----MNEFYKVAAQNGGSIFNEDLTETTINSPENVEALTHLTNEVTDSKVAPSPADLSGQLPEDLFMNGQIAMLHTGIWLFDMFQDA--PFKWDVQVEAGNTQKATHFFANGIGVSKD--SDKKEAAFKFASFMSANEEAAKIRID-NNWELPATENKEILQPYLDATP-PDNRE---AVFESLQYMVL--PPVVKDWNK---I--SDYTNSEFEKVLNGDSTPEKALKNSEDNI-

General information: TITO was launched using: RESULT: Template: 5F7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2030 75428 37.16 203.31 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 37.16 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_5F7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F7V-query.scw PDB file : Tito_Scwrl_5F7V.pdb: