Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence YECYVSLKINGIEHYKPEGRMEFPGATTVYDIGGYGTVVIVLGDTCEPIYKTELKLPQAFDFSSRIFKKRKQSEGQPSGREEVKHQRIEAQEWKPGNV---------------------------------------------------------------------------------------------------------------------- 1JCF Chain:A ((141-336)) -----------LNVEEPSG-------NMVVDIGGGTTEVAVISLG-SIVTWESIRI-AGDEMDEAIVQYVRETYRVAIGERTAERVKIEIGNVFPSKENDELETTVSGIDLSTGLPRKLTLKGGEVREALRSVVVAIVESVRTTLEKTPPELVSDIIERGIFLTGGGSLLRGLDTLLQKETGISVIRSEEPLTAVAKGAGMVLDKVNILKKLQGAG

General information: TITO was launched using: RESULT: Template: 1JCF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 200 -9514 -47.57 -121.97 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -47.57 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_1JCF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JCF-query.scw PDB file : Tito_Scwrl_1JCF.pdb: