Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNHENYWTFCPHNLFWIDRKINRIDMSQTTQTDIITLGDVATRLPSFIPKVPHILNGLKQAYLRTSNTPTGLGIAFEKAVKRNPKGTALLFE------DQSYSYEALNEWANQISHYYLSLGARKGDVIAVMIENRSELIATIVGLAKIGVTIALVNTSQVGKVLAHSINLVKPIAVIAGEEVRAAIDEI---------RQDLNVPKDRFHWFADQATRQKSGTAPKGYVNLADQIDQFPKFNPSTTRSVTGNDGLFYIYTSGTTGLPKAVIFKHSRWTLAYG-TYGHILNLGPDDVMYVTLPLYHATGVVVCWCGVIAGSATLAIRRK----YSTSAFWKDVQKFNASAIGYVGELCRYLMDAPVTE--LDRNHRVTKMI--GNGMRPNIWDKFKQRFG--VKEVLELYASSEGNVGFSNIF-N----FDNTVGFSPTPYAIIQFDKEKNEPIRDKNGWCQKVKAGEVGLLIGKITSR--SPF-DGYTDPEKNKSVIWKDVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWKGENVSTTEVENMVCEYDKIAEAVVYGVEIPNTNGRAGMAAITLADGE-EL--NDADLTEMVTVFKKCLPTYAVPVFLRVQKKVETTGTFKYQKNKLKEEAFNPSKTSERLLALLPGASSYCDITTEIFDNIQAYKYRF 5VPV Chain:A ((118-670)) -----------------------------------------------------------------------AAYNCLDRHYYKNPKKTAIIYEADEPSESREVSYEELMQETCRVANVLKSYGVKKGDAVSIYLPMTWQAAAAFLACARIGAIHSAVFAGFSAESLRDRVNDCECKVLITTDEGRRGGKTIATKQIVDAALQQCPLV-ENVLVLRRTGNK-VP-MTEGRDKWWDEECAKMP--AYCPCERMASEDPLFILYTS---GKPKGVVHSTAGYLLGTALTLKYVFDAHPDDRFACMADIGWITGHSYIIYGPLANGITTAVFESTPVYPTPSRYWDFVDKWKATQLYTAPTAIRLLRRMGEDHVKNHDLSSLRVLGSVGEPINPEAWHWYNDFAGKNQCAIVDTYWMTETGSISIAPLPGAISTKPGSATFPFFGMDVDIIDPQ---------TGQVLEGNDVEGVLVA--RRPWPSIARTVYRDHKRYLETYM----KPYPGYFFFGDGAARDYDGYMWIKGRVDDVINVSGHRLSTAEVESALILHKGVAETAVVGCADDL--TGQAVYAFVTMKPEFD-TKEADLSKELAIQVRKVIGPFAAPKKIYLVSDLPKTRSG-IMRRVLRKIVAGEG----------------------------------

General information: TITO was launched using: RESULT: Template: 5VPV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3012 -15311 -5.08 -30.20 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -5.08 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_5VPV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VPV-query.scw PDB file : Tito_Scwrl_5VPV.pdb: