Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFQVSTLSQKNHVIIQITDTHLLEYPQLEF-----------------------VGMNPEESFHAIIQQILKQ-HPEADAIIHTGDLAQAPTPI---------TYKRYINFMQT--LGLPFFQTLGNHDNVDHFP-------------L----HNEN-------HQQPVVVGL---GNWRVIMLNSAVKGK-----VDGHLSSEQLENLANLLEQF--ADHPVLLACHHHPFAMKSK-WIDHHKLQNSNALLDTLSPFQN-VKALVCGHVHQDSLNIWQ---GV---EFFSTPSTSVQFKPFSNDFALDQNAPGYRYIRLNNDGSFETKVFRLENFKTRINTDISGY 5EBB Chain:B ((2-310)) ---------PAIGQFWHVTDLHLDPTYHITDDHTKVCASSKGANASNPGPFGDVLCDSPYQLILSAFDFIK-NSGQEASFMIWTGDSPPHVPVPELSTDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPVKSV----LEKQTNNPGIRLFQYDPR------------------------

General information: TITO was launched using: RESULT: Template: 5EBB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1121 26329 23.49 113.48 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 23.49 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_5EBB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EBB-query.scw PDB file : Tito_Scwrl_5EBB.pdb: