Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVKNSPAELSSALVNQRNDVPLIRYSGRFAPSPTGPLHFGSLITAVASYCDARAHQGRWLVRVEDTDIPRIYPGSEDHILASLEAFQFDPDAEI--------IFQKDRLDIYESVLDQLKKEGLVYACQCTRKMLGSNAIYAGTCRDLQLD--------FQHQAIRVKVQD-QPICFDDRLQGLHCSNLEHDLGDFVLKRRDGIINYQLAVVADDYLQGITHVVRGADLLDNTERQIWLGQLLGYPKLSYMHLPLAMNDQGQKLSKQNLAHALDLTKA----PELLQQAIQALGQPQVDL-ARPEVMLKQAVTQWNVDLIPHGQQLCGTYL 5TGT Chain:A ((10-322)) -----------------------TTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIEDTDQLRSTRESEQQIYDALRWLGIEWDEGPDVGGPHGPYRQSERGHIYKKYSDELVEKGHAFTCF----------------MHLPKDEVQRRLAAGESHVTRMKVPTEGVCVVPDMLRGDVEIPW-DRMDMQVLMKADGLPTYFLANVVDDHLMGITHVLRGEEWLPSAPKLIKLYEYFGWEQPQLCYMPLLRNPDKSKLSKRKNPTSITFYERMGYLPQALLNYLGRMGWS---EKFTLAEM----IEHFDLSRVSLGGPIFD---

General information: TITO was launched using: RESULT: Template: 5TGT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1197 -7607 -6.36 -29.26 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -6.36 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_5TGT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TGT-query.scw PDB file : Tito_Scwrl_5TGT.pdb: