Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIKEKTLDDLLNSTYTFILENGDHITAS-KGENFEVRNAVLTLENPRSRLSISE-TRSKLISCIGEFFWYLNGSDSLEFIEYY-------ISDYRKY-IEYQIDE----NKPALGAYGPRIFGA--QNQFNKIIEVLKDKPTSRRAVISIYSKDDLLREDSRDIPCTCLLQFFIRNERLHLTATMRSNDAAIGLVHDIFSFTLIQELLLAKLIRYIPNLQLGEYTHIVGSLHIYKKI 1TIS Chain:A ((13-222)) --------------------ENGYETDDRTGTGTIALFGSKLRWD-LTKGFPAVTTKKLAWKACIAELIWFLSGSTNVNDLRLIQHDSLIQGKTVWDENYENQAKDLGYHSGELGPIYGKQWRDFGGVDQIIEVIDRIKKLPNDRRQIVSAWNPAELK--YMALPPCHMFYQFNVRNGYLDLQWYQRSVDVFLGLPFNIASYATLVHIVAK-----MCNLIPGDLIFSGGNTHIYMNH

General information: TITO was launched using: RESULT: Template: 1TIS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 765 -22239 -29.07 -114.63 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -29.07 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_1TIS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TIS-query.scw PDB file : Tito_Scwrl_1TIS.pdb: