Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRCNVMKILIIDNYDSFTFNLYQLLGEILISLKSRKEISDFEIVVKRNNEISLDEIRKLEFDRIIISPGPGSPDDERYFGISKQVI-LQLGKEIPILGVCLGMQGIAYCFGGKVIRAKLPMHGKISTINHSDSGVFSGIPQDVEVMRYHSLIVDSSTLPECFIVTARVNSAYSDSEEEIMGIKHQTYPIEGVQFHPESFASEAGKTLLQNFVHSKL 2A9V Chain:B ((13-200)) ----MLKIYVVDNGGQWTHREWRVLRELG-----------VDTKIVPNDID-SSEL--DGLDGLVLSGGAPNIDEE--LDKLGSVGKYIDDHNYPILGICVGAQFIALHFGASVVKAKHPEFGKTKVSVMHSENIFGGLPSEITVWENHNDEIIN--LPDDFTLAASS------ATCQVQGFYHKTRPIYATQFHPEVEHTQYGRDIFRNFIGICA

General information: TITO was launched using: RESULT: Template: 2A9V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1115 -77230 -69.26 -412.99 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -69.26 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_2A9V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A9V-query.scw PDB file : Tito_Scwrl_2A9V.pdb: