Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNISDNTPQGQLSSQQFYRQYSANALLPELDWQSIFPHSKLSETHIKALNTIYQCAVPLALNVFHDLNFDVFAPAAYHPQGLGLFDKLAQQEENFLKVLTAESENLDHETRHQIWSMLLRSGAVLVFKAWLGHVKLGENQLDMTQFDELSDLLFIKTPPHQLAERLHVDEKAKLDHIFLMYGNDIFLDR-FNSLETVALF-----V--------DLGVYDAAFL-------------------SLRDDCVAEYLKSKGYVTQEQIDDLQCALNPLYCDSLTPKQDCLA 3HZS Chain:A ((101-205)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------AHRVEKQYNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAILASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAM-------------------

General information: TITO was launched using: RESULT: Template: 3HZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 201 -1816 -9.03 -25.22 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -9.03 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_3HZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HZS-query.scw PDB file : Tito_Scwrl_3HZS.pdb: